Journal: ACS Chemical Neuroscience

Article Title: Neurotropic activity and safety of methylene-cycloalkylacetate (MCA) derivative 3-(3-allyl-2-methylenecyclohexyl) propanoic acid

doi: 10.1021/acschemneuro.0c00255

Figure Lengend Snippet: HU- MCA-13 induced neurotropic effect in different neuronal cultures. Neurotropic effect was measured after treatment with 5 μM of HU-MCA-13 of rat PC12 dopaminergic neurons (7 days, top row), mice dorsal root ganglion (DRG) explants (5 days, middle row) and rat spinal cord sensory neurons (14 days, bottom row). The negative control cultures were treated with 0.1% DMSO and the positive control cultures were treated with 50 ng/mL mouse β-nerve growth factor (NGF) for the same periods of time.

Article Snippet: Tissue culture grade mouse β-NGF, was purchased from Alomone Laboratories (Jerusalem, Israel).

Techniques: Negative Control, Positive Control

Journal: Translational Vision Science & Technology

Article Title: Xeno- and Feeder-Free Differentiation of Human iPSCs to Trabecular Meshwork-Like Cells by Recombinant Cytokines

doi: 10.1167/tvst.10.6.27

Figure Lengend Snippet: Xeno- and Feeder-Free Differentiation by Recombinant Cytokines

Article Snippet: Human iPSCs were then cultured in stage 1 differentiation medium containing MEM-α, 10% KnockOut Serum Replacement (Thermo Fisher Scientific) and 2 ng/mL TGF-β 1 , 100 ng/mL NGF-β, and 2 U/mL EPO (Sino Biological, Beijing, China) for 7 days.

Techniques: Recombinant, Concentration Assay, Knock-Out

Journal: Translational Vision Science & Technology

Article Title: Xeno- and Feeder-Free Differentiation of Human iPSCs to Trabecular Meshwork-Like Cells by Recombinant Cytokines

doi: 10.1167/tvst.10.6.27

Figure Lengend Snippet: Xeno- and feeder-free differentiation with recombinant cytokines. ( A ) Diagram of the new differentiation approach with two distinct stages. The first stage is to induce iPSCs to differentiate for 7 days by using TGF-β1, NGF-β, and EPO. Two more recombinant cytokines were involved in the differentiation of the second stage (14 days), including PGF2α and EGF. ( B ) ELISA analysis of the cytokines in the medium conditioned by HTM cells. Observable levels of TGF-β 1 , NGF-β, EPO, EGF, and PGF2α, as the ligands of the selected receptors, were found in the conditioned medium of HTM cells ( n = 3). The experiment was repeated three times by using HTM cells of three donors.

Article Snippet: Human iPSCs were then cultured in stage 1 differentiation medium containing MEM-α, 10% KnockOut Serum Replacement (Thermo Fisher Scientific) and 2 ng/mL TGF-β 1 , 100 ng/mL NGF-β, and 2 U/mL EPO (Sino Biological, Beijing, China) for 7 days.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay